SOLIScript® 1-step SolisGreen® Kit
SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel. Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms). The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube.
Kit Components 40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor 5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye